Your Top Requested Plasmid in 2015!
Type
Blog Post
Published
Dec. 30, 2015, 3:30 p.m.
...Addgene repository in 2015 was...
lentiCRISPR V2
LentiCRISPR v2 originates from Feng Zhang’s lab at...and how does it work?
LentiCRISPR v2 purpose
As its name suggests, lentiCRISPR v2 is a lentiviral transfer...20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high...high titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...pooled library screens.
Availability and Use
LentiCRISPR v2 is available from Addgene both as a single .../Cas9. If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly...kindly provided by the Zhang lab.
Even though lentiCRISPR v2 has only been available for a little over a year...