... Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR... Cas9. Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...popular plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette...and an envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find...wide range of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB...Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian ... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...

... second vector that delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically...typically express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...