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  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (...founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab ...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini...top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid... producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the Trono lab that contains the...
  2. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    Published
    Feb. 6, 2025, 2:15 p.m.
    ...pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particles...for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster,...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...
  3. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those...those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and expression...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab are...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome...
  4. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...the transfer plasmid, a plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral... cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...regions of the transfer plasmid.   Lentivirus resources Browse lentiviral preps available from Addgene...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation by precipitation...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR.... coli. In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits.   ...
Showing: 1 - 4 of 4 results