We narrowed to 40 results for: levi
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TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission. Though the lab ...editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed...
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CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....10.1038/s41587-020-0535-y Kim, Y. B., Komor, A. C., Levy, J. M., Packer, M. S., Zhao, K. T., & Liu, D. R....nbt.3803 Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram...s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m...., Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m..... J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer, J. A. M., & Liu, D. R. (2022). Programmable.... B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m..... B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m..... B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....Commun 8: . https://doi.org/10.1038/ncomms14500 Levy JM, Yeh W-H, Pendse N, Davis JR, Hennessey E, Butcher... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m..... J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer, J. A. M., & Liu, D. R. (2022). Programmable... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m.... AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....00938-z Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....Martinez, R. A., Fong, O., Yao, S., Mortrud, M., … Levi, B. P. (2021). Functional enhancer elements drive... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... embryonic, stem cell-like, or cancerous it is (Levin et al., 2017). These states can even be necessary...modulating bioelectric states in vivo. For example, the Levin lab from Tufts University has used Arch (green-light...optogenetic and GEVI tools (as discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically...and even artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future...PMID: 26988909. PubMed Central PMCID: PMC4991402. Levin, Michael, Giovanni Pezzulo, and Joshua M. Finkelstein...PubMed PMID: 28633567. Mathews, Juanita, and Michael Levin. "The body electric 2.0: recent advances in developmental... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS...and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating....org/10.1038/nmeth.1808 References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques for Manipulating... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m.... CRISPR genome editing experiments. We aim to alleviate this frustration by producing, titering, testing...cells, and, finally, conduct the screen. To help alleviate some of this labor for our users, we now provide... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m...., such as primary cells. Using RNPs can also alleviate difficulties with protein expression that occur...experimental systems of interest. Using this method can alleviate many issues that may arise when using plasmid ... -
2021 Deck the Lab Winners!
TypeBlog PostPublishedJan. 25, 2022, 2:15 p.m....Van Andel Institute. Krampus was created by Nate Levine. View this post on Instagram ... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....coming next. Goal setting and career planning help alleviate the anxiety of graduate and postdoc training. ... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m.... fragment within the gene or loss of the gene alleviates its toxic effect. Negative selection is sometimes... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m.... Mizushima, Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian autophagy research." Cell... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....molecules they were using. SnapGene was created to alleviate these problems through good software design. But...