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We narrowed to 31 results for: lin tian

Showing: 1 - 20 of 31 results

  1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian”) is a postdoc at Harvard...Brandán Pedre Pérez Elizabeth Hubbard Haoyi Qiu He Tian Every two years, we seek applications for awards...engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput screen for... to identify the mutants for further enrichment. Tian notes that this platform can be used to engineer...types of biosensors.   This screening method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived...neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged voltage indicator...indicator expressed in mouse cortex. Image from He Tian. Congrats again to all the recipients! ...

  2. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...pAAV-CAG-RdLight1 AAV9 Biosensor Tian New viral vector pAAV-hSynapsin1-RdLight1 AAV9 Biosensor Tian New viral vector...deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (AAV9), which contains...vector pAAV_hSynapsin_psychLight2 AAV9 Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S...reports from other users. And don't forgot to use the link below to keep up with the latest in Addgene's viral...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s lab has developed dLight1, a molecular tool to...  Using dLight1 sensors in experiments The Tian lab used dLight1 both in brain slices and freely behaving...Feelings of delight associated with the brain’s reward circuits are generally linked to dopamine (DA)...exhibit fast kinetics (10 ms on and 100 ms off), enabling detection of individual peaks of DA release.  ... neurons in live mice Report modulation of DA signaling during learning and reward consumption in live...

  4. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...serotype pAAV-CAG-FLEx-iSeroSnFR AAV9 Biosensors Lin Tian New viral prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors...reports from other users. And don't forget to use the link below to keep up with the latest in Addgene's viral...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...10.1089/hgtb.2019.276. PMID: 31752513. Zhou, Q., Tian, W., Liu, C., Lian, Z., Dong, X., & Wu, X. (2017...the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that give the feature...-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines the orientation of the ITR...and flop orientation. The ITR contains three palindromic sequences (A-A’, B-B’, and C-C’ regions) and ...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Frontiers in molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity...teeth. It is an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because...are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin C). Their fluorescent...aberrant binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium ...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...viral preps here AAV-CAG-dLight1.3b from the Lin Tian Lab is now available as a ready to use viral prep...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...target footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates HR What...Resources and References References Yu, Y., Guo, Y., Tian, Qigi, Lan, Y., et. al. An efficient gene knock-... Sci., 21(18): 6461 (2020). 10.3390/ijms21186461 Lin, S., Staahl, B. T., Alla, R. K., Doudna, J. A. Enhanced...breast and ovarian lines commonly used in lab. It is recommended you check your cell lines for HR-related ...road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you covered in...engineering The easiest way to make a knock-in cell line is to utilize the built-in repair pathway cells ...maximize HDR events, ensure cells are actively cycling through the cell cycle stages. If cells are overly...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...from recent months: Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1. The GCaMP8...enhance axonal labeling and synaptophysin-tagged mRuby to enrich pre-synaptic labeling. Now available ...This approach can augment viral surveillance by enabling rapid assessment of antigenic drift.    Starr...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue their work by...photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

  11. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann, M. (2023)... the gRNA and scaffold. If you don’t feel like doubling the amount of cloning, another option is to use...doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis...Jabaudon, D., & Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, ...

  12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... Central PMCID: PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation...Therefore, the localization of critical fusions (e.g. tubulin or connexin) has been used as a proxy for dimerization...mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short In the early days of GFP applications...protein. Thus, scientist began using relatively long linkers between the protein of interest and the fluorescent...small, non-aliphatic amino acids. However, long linkers increase the chance that a fusion protein is cleaved...victoria GFP sticks out and can be considered a linker (figure 1). In fact, for several FRET biosensors...Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction enzymes...

  13. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...0513-4 Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA (2018) CRISPR-Cas12a target...specific nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological ...be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection)...points out that the protocol is not approved for clinical use at this point, they hope that the protocol...mixed population for an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences...Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ..., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar, J., Osborn, M. J., & Liu....e26. https://doi.org/10.1016/j.cell.2023.07.039 Lin, Q., Jin, S., Zong, Y., Yu, H., Zhu, Z., Liu, G.,...been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods... In addition to the numerous innovations that streamline the process of prime editing, the Liu lab contributed... 3′ end, the Liu lab appended RNA pseudoknots, calling this longer RNA an “engineered pegRNA” or epegRNA...long way to go before we see prime editors in the clinic. We look forward to following new advances in this...

  15. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    .... Many thanks to our Guest Blogger Kristian Laursen! Kristian Laursen is a researcher at Cornell University...This post was contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis...suggests that the final PCR products are pieces of linear, double stranded DNA with homologous ends. These...specified by the arrowheads in (A). Experimental guidelines Primer Design As a rule of thumb, 11 bp of complementary...reaction. Ideally, your primers should be free of palindromic and repetitive sequences, but if present, a minor...the PCR generates a nicked circle rather than a linear product (see figure). Template A high purity plasmid...of the DNA template, and may also decrease the annealing temp of the primers. Because you will be using...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...a000414 (2010). Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612-...infected lung tissue, a Danish biologist named Christian Gram realized that certain stains were retained...hardydiagnostics.com/wp-content/uploads/2016/05/Hans-Christian-Gram.pdf Smith, A. & Hussey, M. Gram Stain Protocols...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...4. Xue, Lin, et al. "Imaging and manipulating proteins in live cells through covalent labeling." Nature...fluorescent proteins. In-cell labeling strategies The majority of in-cell labeling strategies have two parts... protein (Figure 1f). Labeling strategy pros and cons All of these labeling strategies have trade-offs...Overall, self-labeling tag systems (Figure 1b) are perhaps the best method for live cell labeling and the easiest... biochemists have developed techniques to make labeling chemistry easier and more functional in complex...favorite protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good... imaging, the initial breakthrough for in-cell labeling techniques was provided by Roger Tsien, who showed...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...good manager? First, it takes an open mind to be willing to learn and grow in skills. Managing a team is...factors that most contribute to job satisfaction: A feeling of being appreciated and valued This often stems... scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology article “...may not necessarily be comfortable with imposed deadlines (there’s that need for flexibility, sometimes ...

  19. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell University, is a bit old ...something great to offer, I just need to get back to emailing (hopefully non-vacationing) scientists. You can...

  20. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...ubiquitous, making them a rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity...transfection systems using Agrobacterium (such as Nicotiana benthamiana or Arabidopsis thaliana (Xing et al...of the photoswitchable proteins) fused to mVenus linked to a mitochondrial localization sequence TOM20 ...
Showing: 1 - 20 of 31 results