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We narrowed to 33 results for: luciferase

Showing: 1 - 20 of 33 results

  1. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ... plasmids expressing luciferase Luciferase reporter assay The typical luciferase-containing plasmid is...cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic reporter...Well-established Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus...second most commonly used luciferase. Other organisms from which luciferases have been cloned from include...containing luciferase and the gene of interest permits translation of functional luciferase, until miRNA...promoter linked to luciferase. Activation of the pathway will result in luciferase expression and more...Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits ...sources. Unfortunately, detecting traditional luciferases’ dim light emissions requires long acquisition...

  3. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology...transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...need for an excitation light source. Instead, a luciferase substrate like coelenterazine is supplied in ...eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition... luciferase...

  4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...et al. Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...mechanosensor called PILATeS, Pulling-Induced Luciferase Association Tension Sensor (Zhong et al., 2024...sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the I10 domain of the human titin...tension. When this happens, BiT and the LgBiT luciferase fragment (expressed separately) assemble and ... tension sensor. The LgBiT (cyan) fragment of luciferase binds to the BiT Tag (also cyan) embedded in ...systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable for live... B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent ...

  7. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... the split-luciferase system, intein-mediated splicing reconstitutes functional luciferase only when fusions...target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with... fusions of the two halves of the luciferase protein interact and enable intein splicing (5). Screening... Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical probe for detecting protein-protein...

  8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify...single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start...to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start signal...

  9. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...Once we were on the right track, we switched to luciferases to more easily quantify the effect of the different...GUS::tMAS to confirm transformation success. Luciferases are great quantitative reporters and use a substrate...substrate to produce bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then...

  10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image... of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and other enzymes...fluorescent proteins, antibiotic resistance, and luciferase. References Gaudelli NM, Komor AC, Rees HA,...

  11. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...

  14. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  15. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular...

  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...biosensor system was developed by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ...

  19. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used today.  ...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized... assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP...
Showing: 1 - 20 of 33 results