...signal. Prior to the development of these probes, fluorophore binding aptamers and MS2-binding fluorescent ...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...expresses eGFP to indicate reporter abundance, and mCherry fluorescence is activated when APOBEC-Cas9n-UGI-mediated...activity edits out a frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies...efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence...

...pAAV-hSyn-hChR2(H134R)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) ...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a ... a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses nuclear EGFP...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1...

...expression of transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent...interest. The green fluorescent protein (GFP) and mCherry, two of the most common tags currently used, are...used a construct in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, ..., we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles, ...proteins Given the limited expression of hM4Di‐mCherry at the plasma membrane in monkey neurons, we next...small change made a big difference. Replacing the mCherry with the HA tag resulted in abundant expression... of the transgene of interest. In our studies, mCherry interfered with the transport to the membrane of...

...specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The...that turns BFP expression off, while turning on mCherry expression. For this FLEx switch to successfully...sequence in the sense orientation, followed by the mCherry coding sequence in the antisense orientation (Figure... switch will express BFP in the absence of, or mCherry in the presence of Cre recombinase.   Once ...

... you'll need to pick a fluorophore for each target and ensure the fluorophores, together, are able to ...outlining a few commonly used fluorophores and their emission spectra. Some fluorophores carry their emission ...and RB545. Fluorophores can also come in combination of two conjugated single fluorophores, like APC-Cy7...respective colors of a few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note...individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores. A fluorophore's...voltage, the concentration of the fluorophore, and whether that fluorophore appears alone or in conjugation...conjugation with another fluorophore. The brighter a fluorophore, the better the signal-to-noise ratio, and the...

...scFvs) (Inbar et al., 1972) are fused to mature fluorophores, and can bind and light up epitope tagged proteins...colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells... other fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. ...

... are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents permeate the molecular...known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment process used to evaluate...hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO shares many structural...In addition to these established RNA aptamer fluorophores, more are in development, including Carrot and...Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling...polymerase III transcription using a photostable RNA–fluorophore complex." Nature chemical biology 13.11 (2017...on the Addgene blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find...

...), tagRFP (pKa = 3.1) and mCherry (pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown... bind specifically and with high affinity to fluorophores. Examples are the FlAsH tag, the His tag and...enzyme to covalently label the peptide with a fluorophore. Both systems are a hybrid between a genetically...

... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm...

...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...traditional homologous recombination methods). By using mCherry for selection of sgRNA plasmid transformants, they...with the sgRNA cassette on a plasmid expressing mCherry and resistance selection marker HygR. The CRISPR...disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying ...

... ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin...mito-BFP mCherry-Drp1 (GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A... fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1...photon energy is transferred to the acceptor fluorophore, a circularly permuted Venus. In the case of ...of pHlash, the signal from the fluorophore is pH-dependent. Signal is simultaneously acquired from the ...Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or...one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize simultaneously...

...mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F / N99K / S148L / I165V...UV-Violet or blue Green/ Red 4,500 High 553/ 573 PA-mCherries Monomer UV-Violet Dark/ Red >3,000 Medium 570/... of the technique relies heavily on the same fluorophore being imaged multiple times in order to reconstruct.... 2.Subach, Fedor V., et al. "Photoactivatable mCherry for high-resolution two-color fluorescence microscopy...

... al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and...reporter activation and silencing of factor-linked mCherry). They additionally discovered that monocistronic...last month, Robert Campbell’s lab added two new mCherry variants to the repository. These variants can ...ONE paper for more on the directed evolution of mCherry and the spectral properties of various RFPs. For...Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of...influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be...two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with further ...

...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...and named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was...Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...AAV 74302 pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA Zebrafish Expression   Human kinase domain...addition, some versions of the mAID vectors come with mCherry2 or mClover fluorescent proteins allowing you to...