...characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (accelerated...compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H with M164H...compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants...New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed ...synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate...protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat...Methods. 2017. PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl...

... other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I acts as a monomer...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...

...cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...protein of interest can be challenging. In this blog I discuss strategies to generate fusion proteins and...interest and the fluorescent protein. These were either (i) coded by the multiple cloning site and therefore ...often use terminology like “C-terminal fusion,” but I find this very confusing because it can mean that ... is at the C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion...

...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for...bf00428249 Chan MCY, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M (2006) Structural...

... of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...

...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...

...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...

...animals than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is...responsible for Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing...germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice...Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small ssDNA, and... with a long ssDNA donor was less efficient using i-GONAD (15%) vs. microinjection delivery (25-67%). ....2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...