...protein-based toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific...toxin-antitoxin systems and their applications MazE/MazF Aside from CcdB/CcdA, there are many protein-based...protein synthesis. The antitoxin MazE, which binds and inhibits MazF, is continually cleaved by the ClpAP...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...pKSCTMF contains the mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked... Stephen K. Burley. "Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition...multiple genetic modifications in the same strain. MazF expression is under control of the AOX1 promoter...

...work interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments...internship but in the end it turned out to be an amazing experience. I am thankful to have been given the...some of the most genuine people who have shared amazing advice with me. Due to my experience at Addgene...

...was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference at...This fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka ...change.  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools...

... Throughout these talks, I oscillated between amazement at how much has been done with CRISPR technology...type of genetic engineering is a testament to how amazing the downstream payoff could be. Though I attended...of this meeting who have given the attendees an amazing opportunity to share their cutting edge science...

...anniversary was about all the amazing years behind us, as much as it as about the amazing years in front of us....makes us… us. “The Addgene not-for-profit is an amazing service to biology scientists and has saved countless...

...coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry group is a good story ...imaged after i.p. or i.v. administration of the furimazine substrate for NanoLuc luciferase. Both GpNLuc...into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours later demonstrated a more...

...and convince people with diverse backgrounds how amazing their idea is. While communication styles in academia...a story - convince your audience you are doing amazing things."  As Sample6 raised more money, the team...

... at our core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) ...alumni Addgenies on our success. Thanks to our amazing team, we also don’t have to worry about being able...

...G-protein Optical (ONE-GO) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses all the components...ONE-GO Biosensors plasmids here! Janicot, R., Maziarz, M., Park, J. C., Zhao, J., Luebbers, A., Green...

... it is. I would really like to reach all those amazing students that want to have a big impact on the ...science. I think their chances of doing something amazing will be equally high as for those who go to work...

...One of these behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can...were less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised...

...neurons that project to a given brain region (Mazarakis et al., 2001).  This method can also allow scientists...Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies virus glycoprotein pseudotyping...

...guidelines can apply to any workplace or job. It amazes me when the most analytic scientists seem to toss... a graduate school lab. All the lab heads were amazing scientists, but the labs were profoundly different...

...., Wu, W. L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. E., & Gradinaru, V. (2017... Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, ...

...depositors and requestors. Thank you all so much for an amazing twenty years of sharing science!       ...

...CRISPR 101 eBook to introduce students to this amazing genome enegineering technology Resources at Addgene.org...

...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...

...learned in grad school was that scientists have amazing stories to share. I spend a lot of time telling...

...organization has grown, we continue to have an amazing turnout for the annual Halloween costume, so I ...