28 Hot Plasmid Technologies from 2015
Type
Blog Post
Published
Dec. 23, 2015, 3:30 p.m.
...-Rab5
BFP-Rab5
GFP-Rab5B
Vacuolar & Budding Compartment
GFP-Rab4B
Mitochondria
mito-BFP
mCherry-Drp1...Structure
Plasmids:
ER
mCherry-Sec61 beta
BFP-Sec61 beta
BFP-KDEL
Rtn4a-GFP (tubular ER)
Microtubules...dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize ...studying mitochondrial fusion using UV light to differentially photo-label multiple mitochondrial populations...same cell. Mitochondrial fusion is typically assayed in living cells by using mitochondrially-targeted ...between labeled and unlabeled mitochondria, indicative of mitochondrial fusion, is quantified. This strategy...accurately target the mitochondria. The synthetic LOC8R sequence enhances mitochondrial targeting of PSmOrange...