We narrowed to 99 results for: mouse c rel

...standard laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria...terminal); 2xNLS (C terminal) 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) 62933...6xHis 6xHis (C terminal); NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal); NLS ...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models... mutation in mouse ES cells, select for the mutation and inject the ES cells into a mouse embryo to create...bacteria. But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained...knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating mice before...

...Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney, J. ...viral yields relative to AAV9. At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB...Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L....Gong, Y., Ng, C., Nammour, J., Maiz, D., Dujardin, S., Ramirez, S. H., Hudry, E., & Maguire, C. A. (2019)... Poterba, T., Boutros, C. L., Balazs, A. B., Daneman, R., Bloom, J. M., Seed, C., & Deverman, B. E. (2019...can also be paired with Cre-expressing transgenic mouse lines to restrict transgene expression to specific...systemic capsids express differently in different mouse strains, a phenomenon that we are calling “strain-specific...

...an ortholog in C. elegans making it ideal to study basic molecular biology processes. C. elegans are also...PMCID: PMC5525645. Mello, Craig C., et al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance..., but there are many more out there. The house mouse (Mus musculus) Let’s start with the most preferred...mammalian model organism of many researchers: the mouse (Mus musculus). Mice have many advantages as a mammalian... model organism for scientists as they have a relatively short generation time for mammals - the time ...that are laid externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists...6 weeks compared to months to make a transgenic mouse line. Over the years the fly has become an ideal...

...HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse neuron...EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with (magenta) and...recognized by reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. ... on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody... as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts...potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage indicator...10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron The majority...

...reproducible rat or mouse rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions...alpha-5 Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG2a Mouse Anti-Integrin...Integrin alpha-L Mouse IgG2a Rat Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG2a...Human IgG2a Mouse Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG2a Mouse Anti-Integrin...alpha-M Human IgG2a Mouse Anti-Integrin alpha 5 [IPI-MFR5] Integrin alpha-5 Mouse IgG2a  Rat   ...IgG1) chimeric antibodies, and others are rat or mouse IgG2a. Additional human IgG1s are also available...function-blocking antibody, αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The...

...the Allen Mouse Brain Connectivity Atlas, a project to map neuronal connections in the mouse brain. For...J.G., Wohnoutka, P., Gerfen, C.R., Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014). A ..., J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser...finemap of neurons in the mouse brain. MouseLight images can be found on the MouseLight website. Together with...overview see Addgene’s AAV guide. AAV particles are relatively small (20 nm) in size which makes them capable...fluorescent marker. Injection into a conditional mutant mouse brain that expressed Cre recombinase only in pyramidal...injected at several hundred sites across the entire mouse brain. Traced neurons expressed a fluorescent marker...

...Vitale, J., Wong, A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang,...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman,...2023; Wu, Asokan, et al., 2006). AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...retinal cells (Issa et al., 2023). AAV11 AAV11 is a relatively recent addition to the toolbox, with the first...Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. ...Efficient Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal ...Adeno-Associated Virus Serotypes After Local Injection in Mouse and Human Skeletal Muscle. Human Gene Therapy, 31(...

...sophistication of mouse transgenics. Everything that has been described is intended for the mouse; however, all...response gene c-fos. For example, using Chr2 stimulation should result in robust increases in c-fos expression...stimulation compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal...[3], hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle...electrophysiology. Concluding thoughts and moving beyond the mouse While it sounds like a lot of steps and a lot of...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species....proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different types of...

...cysteine) pTP955 (3x cysteines) Mouse IgG1 TP886 pTP943 (3x cysteines) Mouse IgG1 TP1107 pTP1112 (1x cysteine... TP1122 (3x cysteines) Mouse IgG2a TP1129 pTP1005 (3x cysteines) Mouse Kappa TP1170 pTP1174 (1x ...require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren...immunodetection? Enter the Görlich lab and their anti-mouse and -rabbit IgG secondary nanobodies toolbox. Nanobodies... Monovalent Monoclonal Only anti- rabbit and -mouse versions are currently available Not commercially...screening, optimizing, and characterizing several anti-mouse and -rabbit nanobodies. If you’re interested, you...Bates, M., & Görlich, D. (2017). A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. The Journal...

...other C-terminal antibodies only recognized the human c-Myc protein and did not recognize the mouse or chicken...bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]...rat IgG1, human IgG2, mouse IgE, goat IgG, (and more!) as well as the original mouse IgG1. And the refinement...sequences within the middle of the human c-Myc protein or the C-terminus, Evan, et al. isolated six different...experiments? Why Myc? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into...This protein had a highly specific antibody to the C-terminal five amino acids, meaning the tag could be...However, substance P has an amide group added to its C-terminus post-translationally, which turned out to...

...Example: ‘h’ is for Human (homo sapiens), ‘m’ is for mouse (mus musculus), ‘r’ is for rat (rattus rattus or...the order they appear in the plasmid and their relative position to the insert. Example, if you have a... immediately followed by its position (number) relative to the start Methionine (Met) followed by the ...resistance, b) the promoter that drives the insert, and c) any other selection markers (for use in other cell...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the...

...work should be done in mouse and large animal models with mutations in human-relevant exons 45-55, rather...CRISPR can treat a genetic disease in a postnatal mouse model, an important proof of concept for future ... when the genome editing frequency is low, the relative abundance of exon-skipped mRNA is high, likely...rather than in the traditional exon 23-mutated mouse model. Potential off-target effects in muscle, as well...editing partially restores dystrophin expression in a mouse model of muscular dystrophy. Science. 2015 Dec 31...vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2015..., Wagers AJ. In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science. 2015 Dec ...

...optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular...of Cre recombinase are used in combination with mouse lines that express Cre only in the cell type of ...neurons and their synapses in a conditional knockout mouse. Tamoxifen injection induces Cre-ERT2 recombinase... before and after the gene knockout in the same mouse.   Scientists always strive for more. In order...gene and expression of a separate gene. In these mouse lines, gene knockout can be induced by Cre recombinase...Kranz, Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48...Cre-Mediated Recombination at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID...

...lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...library from Xiaole Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene. The guides are optimized...) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image from...nucleases identified, functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...the internal control (Fig 1). Because this method relies on the fluorescence ratio between the 2 fluorophores...

...Additional resources on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part 2 Five Popular Model Organisms...(Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (... P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K., Greaver, J., Janssen, C. F., & Lopez-Berestein,...through the proper basic training prior to conducting mouse experiments. Some of these techniques are what we... to pick up with minimal practice after initial mouse training. Other techniques require more skill and...ingestion, such as experimental therapies or pain relief, straight to the stomach. This technique involves...

...References and Resources References Braun, C. J., Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design... customization, and enhanced expression in many mouse tissues. Safety AAV and lentivirus have different...transduction efficiency over lentivirus in many mouse tissues. This will help improve your selection pool...screens have been used to map tumor suppressors in mouse liver, interrogate immune cells to improve immunotherapy...and generate programmable screening platforms for mouse tissues. Your AAVancement could be next!   References...Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P.,...M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B. (2019). High levels of AAV vector...

...antibody, like if you are using an anti-mouse IgG antibody in mouse tissue. Such species-on-species staining...omitted a mouse IgG2a isotype primary antibody but you still observe a signal from your anti-Mouse IgG2a ... mice with the drug rather than rely on the results of a single mouse. Biological replicates help you ... slowing diseases progression in an Alzheimer’s mouse model, then you could treat one group of mice, the...increase the power of the results. In this case, mouse samples from positive (+), negative (-) and experimental...replicates such as multiple mice in each group and C, technical replicates or repeated measurements will...interactions between the sample and the secondary antibody. C, a label control treats the sample to the full protocol...

...the future of mouse work is bright as it is now easier than ever to manipulate the mouse genome using ... (for knock in models). CRISPR mouse model basics To create a mouse model, the gRNA, Cas9, and donor...of using CRISPR for mouse genome engineering From the very start, generating mouse models using CRISPR...fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ESCs. For ...Generating Mouse Models Using CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6... Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long been extremely...extremely useful in the lab – they are relatively small and easy to work with, making them the go-to choice...

...FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well as a chimera (rabbit). ...most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with monoclonal...sequence. Check out Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP  GFP is more...Epitope tags are short peptides introduced at the N or C terminus of a protein that are bound by antibodies...enterokinase, which recognizes the 5 amino acids on the C terminal of the tag. Of the most common tags, FLAG...not at all. The introduction of a tag at the N or C terminus of a protein may disrupt the biological function...a specific application, or they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging...

...R-mAb K58/35R (Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat... Boeckman, J. X., Manning, C. F., Nguyen, J. T., Bechtold, H., Dumitras, C., Gong, B., Nguyen, K., Van..., you have Goat Anti-Rabbit IgG and Donkey Anti-Mouse IgG both with Alexa Fluor® 488) then you can’t use...Goat anti-mouse IgG1 Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG ...-GABA-AR β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG... Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-... J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv...