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We narrowed to 6 results for: nano bret

Showing: 1 - 6 of 6 results

  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... Enhanced Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase...moieties in their Nano-Lanterns with NLuc and called their improved creations Enhanced Nano-Lanterns. These...the furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals...FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase to excite the Venus fluorescent.... 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc BRET reporters enable sensitive in vivo optical imaging...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nature Comm. 7, 13268...

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... at Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase...different contexts. Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to...enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous Nano-lanterns...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear...separates the signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used...explained further in Takai et al. Nano-lanterns and ready-made Nano-lantern biosensors are available from...laboratory has assembled a collection of multicolored nano-lanterns for use in various applications, including...

  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins...analyze cell signaling pathways or miRNA knockdown. BRET for the best of both (fluorescent and luminescent...light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred...approach can test protein-protein interactions using BRET. A luciferase and a fluorescent protein are each...fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein emits light of a different... approach has even been used in biosensors where BRET reports on the change in conformation of a single...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...variants of their Nano-lantern technology, first developed in 2012. The new cyan and orange Nano-lantern fusions...imaging. The Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...two improved Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by ...light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...
Showing: 1 - 6 of 6 results