...with episomal vectors Episomal vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system...virus- a non-integrating RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express...expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector system increases the...blood drawn from multiple donors with non-integrating episomal vectors. PLoS One, 2011. 6(11): p. e27956....an extra plasmid, pCXWB-EBNA-1 is used in the reprogramming cocktail. pCXWB-EBNA-1 lacks oriP and is unable...pCXLE vectors listed below in Table 1 are available at Addgene. Table 1: List of pCXLE episomal plasmids... pCXLE episomal vector system generated by the group is an optimized mixture of three episomal plasmids...

... Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin...replication (oriP) element and the cis-acting EBNA-1 from Epstein-Barr virus. EBNA-1 binds oriP and allows...genomic integration Replicating EBNA1 episome Non-integrating Medium Medium Generates transgene-free and... Plasmid Non-integrating, usually Medium Medium Generates transgene-free and vector-free cells Limited...required. Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing...reprogramming factors. Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires...the plasmid or silencing of the plasmid by DNA methylation. Another downside of using EBNA1 based vectors...