Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
Type
Blog Post
Published
Oct. 19, 2017, 1:54 p.m.
...identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998). FLPe’s performance...CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon optimization resulted...allow temporal control, ligand regulated Cre and FLPe recombinase have been developed. A common means ...binding domain (LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained...