...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our... pyogenes with an invariant sgRNA scaffold and cloning sites for inserting the guide sequence (pSpCas9...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...

...for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...

...Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of the Broad ...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...How would the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates, restriction...), and now Addgene. I just began uploading our cloning protocols to the site and will keep you updated...

... to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes... multiple cloning site to insert YGOI, a bacterial selection cassette for growth and cloning in bacteria...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...