We narrowed to 4 results for: pSC101

...replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself. RepA binds...mechanisms of stringently controlled plasmids pSC101 and ColIb-P9. pSC101 copy number is regulated by iterons. ...mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation in bacteria Before...balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled and has...the bacterial chromosome. Since replication of pSC101 depends on host-encoded proteins, it can be regulated... and IHF. RepA also controls the copy number of pSC101 through a negative feedback cycle. As with DnaA...expression and thereby the overall replication of pSC101 (del Solar et al., 1998). If that’s not enough ...

...* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (...slightly different but well known derivatives), pSC101, R6K, and 15A. Not all origins of replication are...

...colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid...resistant to both. When this proved successful, pSC101 became the first plasmid cloning vector and molecular...

...plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging from 3-100 per cell, ...a 20-fold variation of gene expression. Because pSC101 is not representative of all types of plasmids,...