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We narrowed to 6 results for: pcDNA3 expression vector

Showing: 1 - 6 of 6 results

  1. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the...most cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing YGOI in...is your expression system or environment. The host organism will determine the type of vector that you...as close as possible to the basal expression level, induce expression at an opportune time or go all out...transforming wild-type yeast with an integrating vector expressing a selection cassette with 5′ and 3′ flanking...

  2. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius...APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9-mediated...ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry...both halves of the modified EB1 protein are overexpressed in cells, microtubule growth can be switched...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... are transiently expressed using either AAV backbones or species-specific expression plasmids. You can...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org...be applied to. When it comes to altering gene expression, CRISPR technologies have gradually taken over...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...silencing pathways. Created on BioRender.com.   Expressing shRNAs using plasmids The design of shRNA plasmids...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence... comes to the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic... lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6:2 ...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...the lab used this vector in chick embryos, it also functions in mammalian expression systems.   Christopher...deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core...with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate transcription. A lentiviral vector with... are an additional layer of control over gene expression that go beyond genomic sequence. Dysregulation...modifications are correlated with alterations in gene expression, but causality and mechanisms remain unclear....that help package DNA, were associated with gene expression or silencing. To alter DNA modifications, researchers...well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...-miR30 (CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...
Showing: 1 - 6 of 6 results