...et al., Nat Methods. 2013. May 5;10(6):540-7. pCoofy vectors for optimizing protein expression Under...Sequence and Ligation Independent Cloning (SLIC). The pCoofy series of plasmids contain a variety of N- and ...To clone a sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector ...vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest in ...in SLIC cloning to form the desired vector. The pCoofy vectors contain the ccdB cassette, which is not...