Special Delivery: Fluorophore Targeting for FRET Studies
Type
Blog Post
Published
July 19, 2016, 2:30 p.m.
...biology 370.3 (2007): 530-540. PubMed PMID: 17512008.
4. Fessenden, James D. "Förster resonance energy transfer...they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that can be inserted into the target protein. ...Range (Å)b
Applications
CFP
Lumio Green
Tc: CCPGCC
50
40-72
Intact Cells
Cy3NTAc
Poly His
44
35...
GFP (fluorescein is similar)
Lumio Red
Tc: CCPGCC
48
38-70
Intact Cells
Cy3NTAc
Poly His
63
50...cells
Cy5NTAc
43
34-62
YFP
Lumio Red
Tc: CCPGCC
54
42-78
Intact Cells
Cy3NTAc
Poly His
65
52...