Plasmids 101: Blue-white Screening
Type
Blog Post
Published
June 4, 2015, 1:03 p.m.
...indolyl-β-D-galacto-pyranoside) into galactose and an insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but ...the other hand, intact β-galactosidase produces pigment from x-gal (included in the transformation plate...β-galactosidase to be expressed and process x-gal into blue pigment (16-20 hours). A plate with only white colonies...after the initial overnight incubation increases pigment precipitation, enhances the blue color of negative...