Plasmids 101: Gateway Cloning
Type
Blog Post
Published
Jan. 12, 2017, 3:30 p.m.
.... For mammalian lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the... of Gateway cloning vectors
Vector type
Vector features
Purpose
Donor vector
attP sites for recombination...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...ccdB gene is present in the donor vectors and the destination vectors prior to recombination, and it is...donor vector.
Method B: TOPO-cloning of the desired insert into an attL-entry-TOPO vector. TOPO...Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors’ design...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR Clonase...