Plasmids 101: Gateway Cloning
Type
Blog Post
Published
Jan. 12, 2017, 3:30 p.m.
...Glossary of Gateway cloning vectors
Vector type
Vector features
Purpose
Donor vector
attP sites ... the Gateway cloning method and its advantages.
An introduction to Gateway technology
The Gateway cloning...fragment cloning
You can use Gateway cloning to insert multiple DNA fragments into many vectors at once...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors... generate entry clones by TOPO cloning or by Restriction Cloning
Destination vector
attR sites for recombination...with the entry clone to generate an expression clone
Ready to try out Gateway cloning?
Many scientists...multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone...