...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses...scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that contains...contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments...

...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. ...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...

...Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro (Plasmid...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding...