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We narrowed to 2 results for: plko 1 scramble shrna

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  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative control for...mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector ...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is...particles. It’s capable of inducible expression of shRNA in target cells by the addition of tetracycline/...target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its name ...
  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...item of 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression,...beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones...with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued to see requests...
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