Plasmids 101: Multicistronic Vectors
Type
Blog Post
Published
Sept. 9, 2014, 8:20 p.m.
...MSCV-IRES-EGFP
IRES
Retroviral
pMSCV-pBabeMCS-IRES-RFP
IRES
Retroviral
pMSCV-IRES-YFP II
IRES
Retroviral
pCMMP-MCS-IRES-Puro...pCMMP-MCS-IRES-Puro
IRES
Retroviral
pEF1a-IRES-Neo
IRES
Mammalian
MSCV-IRES-Luciferase
IRES
Retroviral
pWPI... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them.
Why Use multicistronic vectors?
Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from...