Delivery Methods for Generating iPSCs
Type
Blog Post
Published
April 17, 2018, 1:37 p.m.
...leukemia virus (MMLV)-derived retroviruses. Retroviral vectors, such as pMXs or pMSCV, infect dividing cells...safety required for translational use.
Viral Vectors
Retroviral: Some of the first iPSCs were generated ...corresponding endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming...cloning capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods
Viral Vectors
Adenovirus: Adenoviral vectors infect dividing and nondividing...