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  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral ...High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish... C-terminus of a 2A element, leading to separation between the end of the 2A sequence and the next peptide...Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G D V E E N P G P P2A: (GSG) A T N F S L...References: 1. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from...Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (Hum Gene Ther. 2009 Aug;20...table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...
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