Plasmids 101: Multicistronic Vectors
Type
Blog Post
Published
Sept. 9, 2014, 8:20 p.m.
...Lentiviral
AmCyan-P2A-mCherry
P2A
Mammalian
pC5Kan-P2A
P2A
Insect
pUltra
P2A and T2A
Lentiviral
...High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish... C-terminus of a 2A element, leading to separation between the end of the 2A sequence and the next peptide...Peptide
Amino acid sequence*
T2A:
(GSG) E G R G S L L T C G D V E E N P G P
P2A:
(GSG) A T N F S L...References:
1. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from...Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (Hum Gene Ther. 2009 Aug;20...table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...