...from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...plasmid from the Trono lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

...contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol gene encodes three separate... an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G...were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt et ...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single..., Berry, C., Ecker, J. R., & Bushman, F. (2002). HIV-1 integration in the human genome favors active genes...19491821 PubMed Central PMCID: PMC2835240 Wu Y. (2004). HIV-1 gene expression: lessons from provirus and non-...