We narrowed to 6 results for: rela p65

...then recruit additional activation domains (HS1 and p65). Performance When targeting single genes, SAM consistently... as two other potent transcriptional activators (p65 and Rta). These transcriptional activators work in...activators, fusing the epitope tag of SunTag with the P65-HSF activation domains used in SAM. This method has...generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest levels of...construction: it relies on antibody chains, which are relatively large and are not expressed consistently throughout...

...by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (Zhou et al., 2018). ...activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each genome-bound...using CRISPRa to target individual genes can be relatively laborious because multiple plasmids/viruses are...mouse an attractive tool to probe gene-function relationships in defined substructures of the CNS as well ...can be targeted anywhere in the genome, it is relatively straightforward to use CRISPRa to selectively...tools described above make CRISPRa in the brain relatively easy and accessible. There are also other applications...Rochester Medical Center. He studies gene-function relationships in the CNS using CRISPRa as a member of the ...

...fused to a transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-CIB1 fusion...combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65 - it had the lowest background activity in the dark...they fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9. The N-...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...

...experimental transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety... be labeled in a given population is directly correlated to the length of the truncated attP site. The...

...binding protein (a portion of CRY2) fused to the p65 activation domain, which activates transcription ...below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene...although three of the additional domains tested (VP64, p65, and Rta) were capable of increasing target gene ...three domains onto dCas9 to generate a dCas9-VP64-p65-Rta fusion protein (referred to as dCas9-VPR) had...sites with no cross-talk. These pairs are closely related to the lambda phage integrase system which is the...intracellular pH are required in order to determine the relationships between pH changes and cellular events. To overcome...promoter-selection marker or promoter-inducible expression related vectors are also ready for further chaining. Plasmids...

...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...