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We narrowed to 3 results for: retroviral expression vector MSCV-IRES-GFP

Showing: 1 - 3 of 3 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral Transfer...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase... handling and expression of these vectors when used in experiments. Retroviral vectors make up a large...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively ...cloning and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro...thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived from the Moloney Murine...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Element Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP...pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian...Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian ... for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable or...them. Why Use multicistronic vectors? Detecting cells that are expressing your gene, especially if you...want to express multiple genes in the same cell. Although it is possible to drive co-expression by using...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...lentiviral expression of OSKM reprogramming factors: Mouse Expression Vectors Human Expression Vectors...other retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple...leukemia virus (MMLV)-derived retroviruses. Retroviral vectors, such as pMXs or pMSCV, infect dividing cells,... plasmid-based expression requires serial transfection of 1 or 2 plasmids that express the reprogramming... residual expression piggyBac Excisible Medium Medium Generates transgene-free and vector-free cells...harmful viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic...safety required for translational use. Viral Vectors Retroviral: Some of the first iPSCs were generated ...
Showing: 1 - 3 of 3 results