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We narrowed to 48 results for: retroviral vector

Showing: 1 - 20 of 48 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral Transfer...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively ...For research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...enable long-term stable gene expression. Gamma-retroviral vectors are derived from the Moloney Murine Leukemia...

  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered...methods, the following components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA,...viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable. General...lentiviruses may not be efficiently packaged by γ-retroviral packaging systems, and vice versa. Envelope plasmids...lentiviruses, the genes required for γ-retroviral packaging and transduction are not encoded by the transfer...longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...foundation for producing and using retroviral vectors effectively. Retroviral structure   Figure 2:...have been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (...deep understanding of retroviral biology is necessary in order to use the vectors correctly. A solid understanding...structure of a retroviral particle, which is essentially the same for viruses and viral vectors. Key to the...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from...

  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...different. As a result, lentiviral and retroviral packaging vectors are not interchangeable. While these...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral packaging...Lentiviruses are part of the retroviral family. What scientists often refer to as “retroviruses” are technically ...

  6. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov...breakdown the vector components first by virus and then by function. Lentivirus/gamma-retrovirus Lentiviruses...these vectors that aren’t reflected in the common components outlined above for gamma-retroviruses. While...components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated virus AAV’s elements...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors.  Before...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more detailed...lentiviral vectors that are competent for one round of infection (AKA transduction in the viral vector world...life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...lentiviral vectors? There are several reasons why you might choose an IDLV over a lentiviral vector: 1. No...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things ...

  8. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use, considerable...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ... the genome is more efficient. The first retroviral vectors used were derived from the Moloney Murine...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was...

  9. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology and ...guide to viral vectors Find all of our viral vector blog posts Download our viral vectors 101 eBook Additional...been actively investigating ways to improve viral vector production. Whether redesigning viral packaging...membrane When packaging adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...we believe that this approach can be adapted by vector cores and laboratories alike as an easy and inexpensive...Efficient serotype-dependent release of functional vector into the culture medium during adeno-associated...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the...and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find ...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...murine leukemia virus (MMLV)-derived retroviruses. Retroviral vectors, such as pMXs or pMSCV, infect dividing...safety required for translational use. Viral Vectors Retroviral: Some of the first iPSCs were generated ...corresponding endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming...cloning capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...your gRNA of interest into mammalian cells. This vector was the top requested plasmid of 2015. What makes... This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs and was...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...

  14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system ...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...Addgene: Your lab recently developed a retroviral enChIP system using CRISPR technology. Can you describe...Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita...

  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...components of a virus into different plasmids. For a retroviral system this looks like the genetic cargo in one... researcher.   Figure 1: Separation of retroviral packaging components onto distinct plasmids for... contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...classification. Lentivirus (a type of retrovirus) and gamma retrovirus require more caution as they are integrating...common types of viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...biosensor affinity for calcium.”  Primary Vector Type The vector type should describe how your plasmid ...indicate type in “Vector Type Other” column. For example, you may enter “donor template vector”. Cloning...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids ...

  17. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ... lentivirus guide Sign up to receive our Viral Vectors 101 eBook ...

  18. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...conduct your custom experiment. Fly vectors The vast majority of fly vectors are based on p-element, random... to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our...

  19. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for...

  20. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid...
Showing: 1 - 20 of 48 results