We narrowed to 2 results for: sam ms2 p65 hsf1

...incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses...CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional...not been achieved.” That is, until now. Enter the SAM pooled library In a paper published in Nature in ... expresses the activation helper protein. SAM can be combined with a human genome-wide library to activate...Nature paper reports, “Here we have shown that the SAM system is robust, specific, and can facilitate genome-scale...combined with a compact pooled sgRNA library. Our SAM-mediated screens exhibited a high degree of consistency...References & Further Reading: For more information about SAM, including a tool for getting optimized guide sequences...

...inactive Cas9-VP64, a sgRNA with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al...library substantially identified more hits than the SAM library approach in a comparison study screening ...screening for vemurafenib resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...