...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure on the production of retroviral vectors...Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody...18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...

...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly...

... of the Plasmodium falciparum genome. To put this last point into perspective, the Plasmodium falciparum... over 90% in their work editing the genome of Plasmodium falciparum (the parasite that causes malaria)..."Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature...

...proteins Newly developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging...photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038...

...developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein which transitions... sequence enhances mitochondrial targeting of PSmOrange, as well as other photoactivatable proteins like...like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides a novel strategy for assaying the...subpopulations within the same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate subpopulations...

...coined the term iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of...macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner...

... 2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests...

...membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)A dimerizing...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing FP...

...we first visualized our data in various ways. Smoothing our plasmid order data with a 7-day rolling mean...approximation of the effect of COVID-19 on data smoothed with a 7-day rolling mean. While this global...

...sequestering biotin from being absorbed. In 1940 Esmond E. Snell was able to purify the protein responsible... D. S. and R. L. H. “The Discovery of Avidin by Esmond E. Snell.” J Biol Chem 279.e5 (2004). PubMed PMID...

... osteoblasts [21, 22] Cardiomyocytes [23, 24] Smooth muscle cells [25] Skeletal myogenic cells [26] ... et al., Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells....

...the temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from ...curve does rely on the assumption that there is a smooth, single-step transition between ssDNA and dsDNA...

...plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston University have greatly simplified... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

...- but at Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support...

...the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the...

...noticed that the meetings make the lab run more smoothly. With a concrete plan in hand, trainees are motivated...

...Protocols Lab Tips to Keep Your Experiments Running Smoothly and "Gnome-Proof" This is why the “Bricking Science...

...nervous), but I wasn’t. The presentation went along smoothly and was followed with great discussion and questions...

... by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore...

...techniques and tools that helped make my experience run smoothly and can help other teams successfully overcome...