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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 49 results for: smo

Showing: 1 - 20 of 49 results

  1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure on the production of retroviral vectors...Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody...18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...proteins Newly developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging...photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein which transitions... sequence enhances mitochondrial targeting of PSmOrange, as well as other photoactivatable proteins like...like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides a novel strategy for assaying the...subpopulations within the same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate subpopulations...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...coined the term iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of...macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive ...expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant for everyone...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... 2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...sequestering biotin from being absorbed. In 1940 Esmond E. Snell was able to purify the protein responsible... D. S. and R. L. H. “The Discovery of Avidin by Esmond E. Snell.” J Biol Chem 279.e5 (2004). PubMed PMID...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...tropism for lung, liver, vascular endothelial, and smooth muscle cells (Issa et al., 2023). AAV5 capsids ...to transduce skeletal muscle, cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells (Issa et...

  14. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...the temperature drops, single intensity should smoothly increase as the DNA amplicon transitions from ...curve does rely on the assumption that there is a smooth, single-step transition between ssDNA and dsDNA...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston University have greatly simplified... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the...
Showing: 1 - 20 of 49 results