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We narrowed to 5 results for: streptavidin constructs

Showing: 1 - 5 of 5 results
  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8. Since ...control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so ... the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984...direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). Using this ...
  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation (IP), and tandem mass tag...
  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, ...of inducible promoter constructs. Up to 34 building blocks were used to construct 20kb long inserts. The...fused to a streptavidin core that are able to retain reporter proteins fused to the streptavidin-binding ...att sites are supplied as individual reporter constructs or as the fully assembled pMemoryArray.   Yang...Biotechnol Lett. 2015. 37:383-389. Toolkit for constructing fusion proteins with customizable N- and C-terminal... to 5 different elements. Lentiviral vectors constructed with the MuLE toolbox have been used for a variety...cloning method that simplifies the process of constructing specialized fluorescent reporter plasmids with...
  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...Fourier-shell correlation curve (D), and the reconstructed 3D map with 3.1 Å global resolution (E). Figure...
Showing: 1 - 5 of 5 results