... Commercial Plasmid Prep Kit Amount 1000 Lanes 1000 Lanes 1000 Lanes Cost $200-$500  <$10 $20-30 ...convenient, commercial ladders cost between $200-$500 for 1000 lanes worth of ladder. The Penn State ladders are...plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder. Using...Other Resources Read about Gel Stains on the BitesizeBio Blog ...

...larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and the Dark Lord rules ...Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

... but a select few are particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested...requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...

...barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell™ reduces every... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

...and very cool 8,768,560 hours – or almost exactly 1000 calendar years!   *Dammit, man, I’m a scientist...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

...particles. Studies have found that values can be 10-1000 fold higher than for functional assays depending... based off of p24 quantification tend to be overestimates since they can include free p24 and defective...vectors carrying fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our...multiplicity of infection (MOI) to ensure only one copy integrates, selecting by antibiotic-resistance or single...

...coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared to that...biological questions. The two most common uses are: 1) to test the expression level in a specific system (by measuring...occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP). FRET...create the chromophore. This can be from a few minutes after it is translated to a few hours. For example...However, for most FPs it is a few seconds to a few minutes. Photostability can be affected by experimental...greenfluorescentblog.wordpress.com.   More Helpful Websites & Resources: FP guide at Addgene Interactive ...

...Beroukhim and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify...dependencies detected. Given this new information, Misek notes that in theory, if the DepMap analysis had an equal...and references References Germline variation contributes to false negatives in CRISPR-based experiments...Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting genetic ...

... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...tightly regulated. Common promoters for eukaryotes and prokaryotes We have included two reference tables...bacteria this is done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible...expression mRNA Yeast promoter containing Gal4 binding sites, commonly used in Drosophila Specific Requires the...

...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume...detection of the product. As an added benefit, replicates are built into the technology. One well (containing...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...low as 1C/s. Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been ...

...or selection cassettes. This class of template should have homology arms between 500-1000 bps. HDR efficiency...2023. DNA damage drives genome instability and contributes to cancer, premature aging, and other harmful...displacement loop (D loop). The recombination intermediates can then resolve to complete the DNA repair ...a one-ended DSB). This single invasive strand initiates primary synthesis from a template followed by ...sequence to be modified. In general, the insertion sites of the modification should be very close to the ...determinant when selecting a type of donor. ssDNA templates (referred to as ssODNs (oligodeoxynucleotides)...efficiency with circular plasmid templates is generally low; to increase the frequency of edits, researchers...

...includes SpCas9, and shortern orthologs approximately 1000 amino acids in size. From the pool of shorter orthologs... suitability for in vivo studies. Testing AAV-SaCas9 in vivo To test the efficiency of AAV-SaCas9 in vivo...Cas9 genome editing varies across targets, they tested two genes in mice. For both genes, they saw indel...polypeptides that, when at the ends of two proteins, mediates protein trans-splicing, similar to an intron in...proof-of-concept split intein SpCas9 which had modest editing rates in HEK-293T cells when compared to the full-length...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

...design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR...discovery. With these screens, the investigator generates a cell population where all genes are knocked ...groups and, as a consequence, which genes are good candidates for being involved in the studied phenotype. ...These targeted screens can allow a researcher to test which of the differentially expressed genes found... is added), and decide which genes you’d like to test for phenotypic effects. Typically the phenotype ...interest (that were not found by Green Listed), and test if any of the alternative names works better. To...

...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600   Choosing a photoactivatable...be activated from low fluorescent states to high fluorescence states, they can change from one fluorescent... light. The fact that blue light both activates and inactivates Dronpa is not ideal, since this means ...488nm blue light. The T203H mutation in PA-GFP generates a chromophore population that is more neutral,...but then further exposure to blue light also inactivates the chromophore, leading to a non-fluorescent...photoactivation. A high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence...and display bright and photostable green and red states. Beyond these imaging applications PA-FPs have ...

... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this...restriction sites flanking the promoter-gRNA construct, but with different sequences adjacent to the sites. When...genome by removing the sequence between two target sites. Modifying multiple genes at once. Using multiplexed...promoter-gRNA constructs in order to target multiple sites! In this post, we'll cover Cas9 multiplexing options...oligonucleotides. These plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs. When these...unique overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and drive ordered assembly...transcribed together and are flanked by specific sites that allow them to be cleaved and released. These...

...fibroblasts to myoblasts. Cell, 1987. 51(6): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic...13]:  Germ cells [14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial...22] Cardiomyocytes [23, 24] Smooth muscle cells [25] Skeletal myogenic cells [26] Chondrocytes [27...27]         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32...reverts back to a pluripotent state, which then proliferates and is redifferentiated to a different cell ... from donors. Apart from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and...transdifferentiation. Multiple cell types such as fibroblasts, hepatocytes, and pancreatic exocrine cells have been successfully...

...produces point mutations at a rate of up to 1 per 500-1000 bp. In comparison, the DNA replication error rate..., et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from the...availability of enhancer sequences in different chromatin states. Alexander Stark and colleagues at the Research...self-transcribing active regulatory region sequencing) that tests enhancer sequences outside of their endogenous chromatin...Synthetic and basic biologists alike often need to test multiple expression systems before they achieve ...bacteria, the process of cloning, combining, and testing these different parts can be quite arduous. The... lab generated 5 common 30bp overlap sequences (Sites #1-5). Each overlap site encodes a unique restriction...

...sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research, 2016). These events...of the Federal Labor Standards Act (FLSA) which mandates that all workers across the country either be ...specifically chosen to better define productive routes for advocacy efforts and help participants develop...Fisher Scientific Developing effective science advocates is an important way to push the balance from challenge...Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central...from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph F. Pierre and Erin... advocates...

...new target sites. Created in BioRender.com.   Class II TEs: DNA transposons Class II TEs are also ...of transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons.... Class I Transposable elements (TEs): Retrotransposons  Class I TEs are also known as retrotransposons...the TE while also increasing the size of their host’s genome.   There are two types of Class 1 TEs: those...DNA sequences or target sites. Integration of most transposable elements (TEs) results in the duplication...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...encode a transposase enzyme.  Class I TEs are considered replicative since every time they jump they make...