Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: tetracycline transactivator

Showing: 1 - 6 of 6 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ..., Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered... in a similar manner to the Tet system. The transactivator configuration of the system is a positive repressible...the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR to the activation.... Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate their respective...preventing transcription. In the presence of tetracycline (Tet), TetR preferentially binds Tet over the...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response... inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...or off depending on what tet transactivator is used. UAS General expression mRNA Yeast promoter containing...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, ...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...
Showing: 1 - 6 of 6 results