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We narrowed to 21 results for: tifa

Showing: 1 - 20 of 21 results
  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs commonly...it reduces the risk of spectral contamination artifacts, it has better folding and maturation kinetics...it’s predecessor, sREACH, allowing for reduced artifacts due to residual fluorescence. ShadowY was additionally...
  2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...This property of EGFP can lead to significant artifacts. If you're using green fluorescent protein or ...want a fusion tag to be inert, to not produce artifacts in your experiments. Considerable effort has gone...levels of the fusion protein. This may alleviate artifacts for moderately dimerizing FPs such as EGFP, but...studies and greatly lower your chances of finding artifactual results. Many thanks to our guest blogger Erik...
  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...efficiency and specificity. With TurboCas, the Ali Shilatifard Lab was able to identify both new and known regulators...miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created a very quick and efficient way to...Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard, A. (2024). TurboCas: A method for locus-specific...
  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...their findings may be the result of a technical artifact that introduced an ancestry bias into their experiments...attribute to an ancestry group what is actually an artifact introduced by bias within the design of the experiment...
  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...resources References Brown-Harding H (2020) Fixation artifacts and how to minimize them. FocalPlane. https://...focalplane.biologists.com/2020/07/07/fixation-artifacts-and-how-to-minimize-them/  Goldenthal KL, Hedman...
  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...events, the lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only...and significantly decrease the probability of artifacts. Off-target controls A seldom used control for...
  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...detection of proteins and complexes, without the artifacts sometimes associated with plasmid-based overexpression...
  8. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... protein at higher levels, you risk observing artifacts only present when the protein is at a very high...
  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...(7):290-5. PubMed. Is VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001...
  10. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...insert, not necessarily YOUR insert. Any cloning artifact that disrupts the α-peptide DNA will also lead...
  11. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...elegans on extrachromosomal arrays can lead to artifacts due to overexpression. CRISPR can insert the FP...
  12. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...wavelength measurements are more susceptible to artifacts and pH interference, and comparison between experiments...
  13. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... one of several new tools for unraveling the multifaceted role Myc plays in human health and disease. ...
  14. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...occurred. Having too many insertions can cause artifacts from over-expression and it is likely that such...
  15. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... less sensitive to direct acceptor excitation artifacts and it is possible to use a non-fluorescent acceptor...
  16. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Center: An Elaboration of AAS Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is...
Showing: 1 - 20 of 21 results