We narrowed to 12 results for: ubiquitin expression vectors

...The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our...empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian...

...-baToN, “sender” cells express GFP on the surface and “receiver” cells express an anti-GFP nanobody on...response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern and ... nanobody cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...visualized.  The tool-kit consists of highly useful vectors compatible with Gibson and Saptrap cloning systems...for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Jacobs-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance markers...

...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...General expression mRNA Plant promoter from maize ubiquitin gene Constitutive  Gives high expression in plants...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...deacetylation. Ubc General expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  ...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...

...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... 6,000 times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the...plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR... our Viral Production Process Find other Viral Vectors ...

...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read...activation. Vary the expression levels of bait and prey proteins - overexpression can lead to false interactions...During this period, proteins were successfully expressed as fusions that retain their individual activities...see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a reporter...bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI’s interact, this...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...strains are used to select for binding-dependent expression of the HIS3 gene, which is required for histidine...

... I wanted to find a lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting ...categories Expression: Select the model system for your research (e.g. Mammalian) Vector Type: What type...Addgene is currently distributing 94 plasmids expressing validated antibodies for neuroscience research...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

...quantifying variant expression with a minimally disruptive tag. Find HiBiT-TP53 Fusion Vector at Addgene! Following...HiBiT. When EVs from cells expressing ADAR1-HiBiT were added to LgBiT-expressing hepatocytes or co-cultured...low-levels of ectopic expression, thus avoiding artifacts common in overexpression models. Although HiBiT...signal, strong enough to detect from proteins expressed at their endogenous levels. HiBiT is compatible...cell models, and even introduce mutations. This vector-based use of HiBiT provides a means to quickly ...to provide complementary validation of protein expression and localization. HiBiT-tagged targets in translational...plasmids from the Promega Plasmid Collection that express similar constructs available at Addgene. Read the...

...codon site of interest can be cloned into an expression vector with Gibson assembly. While this is a comprehensive...relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a ...selective pressure winnows the pool down to plasmids expressing variants with optimal function. High-throughput...ligand binding assays, phage display assays, ubiquitination activity assays, toxin activity, small molecule...is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase...2 instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...

...Fluorescent Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks...box of tags and fusion proteins to aid in the expression and purification of recombinant proteins. Tags...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding ...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Biochemists and molecular biologists who need to overexpress and purify proteins can face any number of technical... some of the hurdles to overcome in order to overexpress a recombinant protein? It is not generally in...in a cell’s best interest to overexpress a protein. Energy and cellular resources are being spent to make...

...promoter is expressed. Cre-mediated deletion of one or several of these genes permits the expression of genes...activate the expression of a different fluorescent protein. Therefore, each cell could express a unique combination... tamoxifen-inducible ubiquitin background, each cell in the mouse that expresses Cre undergoes exactly...copy of the transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with ...the low expression of XFP of Brainbow by incorporating a Tet-Off system to boost XFP expression. The increased...variety of colors when a combination of XFP are expressed. Each cell remains the same color for its entire...of the downstream OFP, GFP, or RFP. Cells that express the construct but do not undergo recombination ...

...drive gRNA expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved...Cas9 expression is commonly driven by plant-derived RNA pol II promoters which regulate expression of longer...mRNAs for gene expression). Examples of commonly used RNA pol II promoters for Cas9 expression include the...the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin promoters (Belhaj...system in plant cells. Like other model systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter...OsU6 or OsU3, which are commonly used to drive expression of small RNAs in their respective species. Addgene...with other model systems, multiple gRNAs can be expressed to modify several genomic loci at once (get more...

...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic... lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6:2 ...