We narrowed to 93 results for: ple

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...information. To learn more about Addgene's MTA process please visit our Help Center . The MTA Process: Once your...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...

...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ... Cookson 29417 pLenti6-DJ1-V5-E18Q PARK7 V5 CMV Parkinson's Mark Cookson 29418 pLenti6-DJ1-V5-D149A PARK7...ALS Mohan Babu 83444 pLEX_307-SOD1WT SOD1 EF-1 alpha ALS Mohan Babu 83445 pLEX_307-SOD1E133delta SOD1 EF...Mohan Babu 83446 pLEX_307-SOD1E133insTT SOD1 EF-1 alpha ALS Mohan Babu 83447 pLEX_307-SOD1E133K SOD1 EF...

...the following three buffers. All buffers are supplemented with 0.001% Poloxamer 188 or 0.014% Tween 20...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...

...page . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog...for the 20 µL sized testing sample is denoted with a .T suffix (for example, AAV1.T). The catalog ID for...for the 100 µL sized sample does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing...different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are also available...include virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...CRISPR/Cas9 in mammalian cells as an example, but many of these principles also apply to using CRISPR in other...while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs... process of interest. Do you want to: Generate complete and permanent loss of gene expression or function...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites...selecting a gRNA, but you likely won’t be able to completely avoid them. To increase specificity, consider...the genomic region you intend to target in your samples to ensure there are no discrepancies with the reference...produce the Cas enzyme, and the enzyme then forms a complex with the gRNA for CRISPR activity. Because you ...

...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...

... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...

...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the array...zinc fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable... bp. TAL effectors have some restrictions (for example, the target must start with a T), but they still...Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final array is composed of 18 TAL effector...

... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and...understanding the function of your favorite gene. For example, fusing your protein to an epitope tag, such...RFP - A zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral...destination vector, expression, CMV/TO promoter, GFP-Zeo pLenti CMV/TO Zeo DEST - Tet-inducible ...GFP - Retroviral shRNA expression pLenti CMV GFP DEST - Lentiviral Gateway...

... lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting...Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures...specific QC experiments were performed on your lot, please contact us . Full sequencing of the Viral Genome...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified...