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We narrowed to 80 results for: Cre expression vector

Showing: 21 - 40 of 80 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...are available. An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive...Adenosine Express the adenosine sensor GRAB_Ado1.0 in neurons or astrocytes (Constitutive or Cre-dependent...cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent...iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a ...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 ...Mammalian expression of firefly luciferase Lee Helman 131860 pZmUBQ:LUC Firefly ZmUBQ Plant expression of firefly...Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging...

  4. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific...Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent ... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

  5. Bacterial Expression Systems

    Type
    Collection
    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...

  6. Control AAV Preps

    Type
    Collection
    ... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2,...EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2...mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs...placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate TALEN...1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal tal...

  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ...

  9. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...

  10. Plant Plasmids and Resources

    Type
    Collection
    ...designed for creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene...Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on... - Tools for gene and protein expression and mapping of gene expression in plants. Figshare - Plant Illustrations... that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for ...CRISPR Resources for a full selection of plasmids expressing different functional variants of Cas9 and empty...

  11. Antibody Plasmid Collection

    Type
    Collection
    ... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...

  12. Plan Your Experiment

    Type
    Collection
    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...AAV vectors offer some advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  14. CRISPR References and Information

    Type
    Collection
    ...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help...iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and...

  16. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently integrated...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Publication Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...

  18. Microbiology Resources

    Type
    Collection
    ...Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma and... E. coli expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier...- Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Santalucia Labs pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted open...

  19. Lentiviral Prep Service

    Type
    Collection
    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti...

  20. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...
Showing: 21 - 40 of 80 results