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  2. CRISPR Plasmids - Plants

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...

  3. Validated gRNA Sequences

    Type
    Collection
    ... may only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must...used with targets that are upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA ...

  4. Cre-lox system

    Type
    Collection
    ...vector for Sox1 locus CAG Mammalian Okano 153207 pAM-AAV-mSncg-Cre Retinal ganglion cell-specific expression...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Spinocerebellar Ataxia Pietro De Camilli 194242 pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA SNCA HA SAR-CAG ...Parkinson's Michael J Fox Foundation MJFF 194247 pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA SNCA GFP CAG...Parkinson's Michael J Fox Foundation MJFF 194248 pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA SNCA GFP CAG...T7 Huntington's Pamela Bjorkman 11514 pET32a-HD39Q HTT His, Trx T7 Huntington's Pamela Bjorkman 11515 ...11487 pET32a-HD16Q HTT His, Trx T7 Huntington's Pamela Bjorkman 11503 pBabe neo human Frataxin FXN Friedreich...11515 pET32a-HD46Q HTT His, Trx T7 Huntington's Pamela Bjorkman 12165 pSico Dnmt1 DNMT1 GFP CMV Hereditary...NR4A2 NE CMV Parkinson's Shu Leong Ho 102364 pSIN-PAmCherry-mSNCA-NE SNCA NE EF-1 alpha Parkinson's Shu Leong...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression PAmCherry1 564 595 8 6.3 23 min Monomer pPAmCherry1-N1 - Mammalian Expression pPAmCherry1-C1 - Mammalian...pBAD/HisB-PAmCherry1 - Bacterial Expression PAmCherry2 570 596 13 6.2 18 min Monomer pPAmCherry2-C1 - Mammalian...Expression PAmCherry3 570 596 5 6.2 25 min Monomer pPAmCherry3-C1 - Mammalian Expression PAmKate 568 628 ...628 5 5.6 19 min Monomer pPAmKate-N1 - Mammalian Expression pBAD/HisB-PAmKate - Bacterial Expression PAiRFP1...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...monitoring dopaminergic activity in vivo. Nat Methods. 2023 Nov 30. Yulong Li Dopamine Dopamine sensors ...2020.02.07.939504 Loren Looger , J. Julius Zhu Dopamine Red and green GRAB_DA3 dopamine sensors Improved green and red...monitoring dopaminergic activity in vivo. Nat Methods. 2020 Nov;17(11):1156-1166. Yulong Li Dopamine Ultrafast...neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics with ...science.aat4422. Lin Tian Dopamine Yellow or Red genetically encoded dopamine sensor YdLight1 and RdLight1...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(...

  8. Brain Initiative Collection

    Type
    Collection
    ...pAAV-hSyn-GRAB_DA1m express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 115892...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV5 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV9 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125707-AAV9 pAAV-CAG-RdLight1...pAAV-CAG-RdLight1 Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708...

  9. mTOR Pathway

    Type
    Collection
    ...Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...31930 pPAmCherry-a-tubulin Microtubules alpha-tubulin PAmCherry Vladislav Verkhusha 31949 pPAmCherry-b-actin...Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre...-actin Actin Filaments beta-actin PAmCherry Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...primarily involves the malfunction and death of dopamine-producing neurons in the substantia nigra, one...AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent expression... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat Methods. 2020...30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout . Maimaitili...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed...Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections to the nucleus accumbens...2018. Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches...

  14. Retrograde AAV viral preps

    Type
    Collection
    ...genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m...GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors Bidirectional...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Botch Rat Mouse IgG2a 114488 Anti-Dopamine D3 receptor [N331/19R] Dopamine D3 receptor Human Mouse IgG2a ...NALCN Rat Mouse IgG2a 177509 Anti-Dopamine D2 receptor [N186/29R] Dopamine D2 receptor Rat Mouse IgG2a 177510...Gamma-protocadherin-B2 Mouse Mouse IgG2a 222156 Dopamine D2 receptor [N186/26R] Dopamine D2 receptor Rat Mouse IgG2a 222157...

  16. Biosensor AAV Preps

    Type
    Collection
    ...NIR-GECO Acetylcholine Sensors iAChSnFR GRAB_ACh Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA...Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors 111066 pAAV-hSyn-dLight1.1 Syn dLight1.1...

  17. Genetic Code Expansion

    Type
    Collection
    ...unnatural amino acids Mammalian TAG Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial... S. cerevisiae Bacterial Ahmed Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...complement component 3 ARMD9, ASP, CPAMD1 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 ...R33729_1, SF20 C3 complement component 3 ARMD9, ASP, CPAMD1 C3AR1 complement component 3a receptor 1 AZ3B, ..., C3AR, HNFAG09 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 C5AR1 complement component...

  19. Synthetic Biology - Overview

    Type
    Collection
    ...Antonio Richart Herbert Sauro Claudia Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening...
Showing: 21 - 40 of 42 results