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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 38 results for: cas9

Showing: 21 - 38 of 38 results

  2. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require that each DNA target have...

  3. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA....gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification. Various epitope tags...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system . The...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...

  7. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...

  11. Rett Syndrome

    Type
    Collection
    ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...

  12. Bacterial Expression Systems

    Type
    Collection
    ...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa...

  14. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome...

  15. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  16. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your... separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of...
Showing: 21 - 38 of 38 results