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We narrowed to 41 results for: cas9 expression vector

Showing: 21 - 40 of 41 results

  1. CRISPR Plasmids - Base Edit

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    ...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant and... lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, yeast...

  2. CRISPR Plasmids - Plants

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    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009::AtU6p...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in plants. CRISPR...Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (...

  3. CRISPR Plasmids - dCas9-FokI

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  4. Cre-lox system

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    ... EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...Cre-dependent Cas9: Cre-dependent Cas9 can be knocked in to a mouse at the Rosa26 locus to facilitate Cas9 mediated...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...

  5. CRISPR Plasmids - Cascade-Cas3

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target...underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part to the difficulties ...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  6. CRISPR Plasmids - gRNAs

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    Collection
    ...gRNA expression vectors available from Addgene....Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal...

  8. CRISPR Plasmids - Purify Genomic Loci

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...purify genomic loci. Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ...purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  9. Neurodegeneration Research Collection

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    Collection
    ...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base... dopamine research. CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...

  10. Botman-Teusink Yeast FP Collection

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    ...Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection markers for...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013 Jul 2;8(7):e67902...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...

  11. CRISPR Plasmids - RNA Targeting

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  12. CRISPR Plasmids - Parasites

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  13. Distribution to Industry

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    ...while also adding additional filters such as expression, vector type, species, and more. Browse Browse the...CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...

  14. Tetracycline Inducible Expression

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    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Church 87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 ....Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...

  15. Bacterial Expression Systems

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    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...

  16. CRISPR Plasmids - Repress Gene Expression

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    ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...transcriptional inhibition. Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast...Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  17. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... plasmids for activating or upregulating gene expression in various model organisms. CRISPR...transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your...

  18. CRISPR Plasmids and Resources

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    ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  19. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  20. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...to introduce a DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...
Showing: 21 - 40 of 41 results