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We narrowed to 45 results for: crispr cas9 expression plasmids

Showing: 21 - 40 of 45 results

  1. CRISPR History and Development for Genome Engineering

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene has... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...

  2. Brzezinski Lab CRISPR Collection

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    Collection
    ...and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic...Schematic of the CRISPR Collection. Plasmids 159654 and 159655 are single guide expressing double stranded...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...

  3. CRISPR Plasmids - Plants

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    ...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...CRISPR expression plasmids that have been designed for use in plants. CRISPR... CRISPR Plants CRISPR Plasmids: ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...

  4. CRISPR Plasmids - dCas9-FokI

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    ...effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids are available...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  5. CRISPR Plasmids - Cascade-Cas3

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    ...tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...

  6. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... any of these gRNA plasmids and review the publication associated with each plasmid for more information...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...

  7. AAV Molecular Tools

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    Collection
    ...encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru ...pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive...positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent...inducible Synapsin promoter (ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive...

  8. CRISPR Plasmids - Purify Genomic Loci

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    ...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  9. CRISPR Plasmids - RNA Targeting

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    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...

  10. CRISPR Plasmids - Parasites

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    ...CRISPR expression plasmids that have been designed for use in parasites....Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ...than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  11. Neurodegeneration Research Collection

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    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...

  12. Distribution to Industry

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    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...additional filters such as expression, vector type, species, and more. Plasmids Browse the table below to...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...

  13. Cre-Lox and Other Site-Specific Recombinases

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    ...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase...Chemogenetics Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...full catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...

  14. Rett Syndrome

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    ... using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources...MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene. Use the search bar... Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch labs Plasmids MECP2 ...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ...

  16. Botman-Teusink Yeast FP Collection

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    ...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink Lab Materials...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources This comprehensive...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...

  17. Bacterial Expression Systems

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    ...Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and Resources...like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids that ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for ...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...engineering and expressing zinc finger proteins. Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome...

  18. Bikard Lab - CRISPR Repression Collection

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    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The... using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur... has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently...

  19. CRISPR Plasmids - Repress Gene Expression

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    ...below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression in mammalian... CRISPR Interfere CRISPR Plasmids: Interfere...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...

  20. CRISPR Plasmids - Activate Gene Expression

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    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression in mammalian...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...
Showing: 21 - 40 of 45 results