CRISPR Guide
Type
Collection
...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...it meets two conditions: The sequence is unique compared to the rest of the genome The target is present...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...effects Sniper-Cas9 — less off-target activity; compatible with truncated gRNAs to increase specificity ... offer high efficiency and low indel formation compared to HDR. Prime editing requires more optimization...) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-K includes just tnsB , tnsC , and tniQ...