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We narrowed to 99 results for: ple

Showing: 21 - 30 of 99 results

  1. Plan Your Experiment

    Type
    Collection
    ... we will use the example of CRISPR/Cas9 in mammalian cells, many of these principles apply to using CRISPR... process of interest. Do you want to: Generate complete and permanent loss of gene expression or function...major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression...transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex, which is delivered to cells using cationic lipids Transient... will depend on your specific application. For example: To activate or repress a target gene using dCas9...nucleotides within the selected target sequence. For example, gRNA targeting sequences containing a G nucleotide...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and...understanding the function of your favorite gene. For example, fusing your protein to an epitope tag, such...RFP - A zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral...destination vector, expression, CMV/TO promoter, GFP-Zeo pLenti CMV/TO Zeo DEST - Tet-inducible ...GFP - Retroviral shRNA expression pLenti CMV GFP DEST - Lentiviral Gateway...

  3. Viral Production

    Type
    Collection
    ... lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using primers and probes targeting...Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures...specific QC experiments were performed on your lot, please contact us . Full sequencing of the Viral Genome...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral... In these mice the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent...

  5. AAVED

    Type
    Collection
    ...and protocols showing examples of AAV strategies for intersectional control. Please see our list of suggested...publicly, but please only share materials that you are comfortable and willing to share. Please only share...information. Experimental protocols or schematics. Examples of research materials to share: In vivo data: ...Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox link...contributions and your commitment to open science! Please see the meeting preparation section for how to ...

  6. Arf GTPase Family

    Type
    Collection
    ...cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of...ARF GTPase family, information on each gene, and examples of plasmid types available for each gene. Click...gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids ...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375 181 Bacterial... and GEF family, information on each gene, and examples of plasmid types available for each gene. Click...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP) 55738 ...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    .... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found...visualize and track clones of cells over time. Examples of useful tools for studying cells that share ... Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish, ranging ...for studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...targeting the PITCh donor plasmid. Note that the principle of the PITCh system is different from the conventional...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...nuclear and cytoplasmic proteins, which can then be depleted very rapidly after the addition of auxin to the...and can be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual..., to order more than 2 µg of a single plasmid, multiple orders would need to be placed through our website...website. Every order will need to be completed independently including the check out and will require separate...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

  10. Rett Syndrome

    Type
    Collection
    ...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...your plasmids! To get started with your deposit, please click here ....
Showing: 21 - 30 of 99 results