We narrowed to 93 results for: ple

...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral... In these mice the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent...

... seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...most popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a...constructing CRISPR gene-editing vectors in just two simple cloning steps. CRISPR Kate Creasey Krainer , Vladimir...functional variants of Cas9. A CRISPR/Cas9 toolkit for multiplex genome editing in variety of plant species (e....CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Plant...planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC) and Förster Resonance Energy Transfer...MoBiFC Kit Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein...

...Return to top Multiplex gRNA Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA...gRNA plasmids, including: Multiplex CRISPR/Cas9 Assembly Systems Multiple Lentiviral Expression System ...gRNA Expression Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires you to express both ... for expression of gRNA and Cas, gRNA only, or multiple gRNAs in the tables below. Looking for non-targeting...Selectable Marker PI Publication Return to top Multiplex gRNA Plasmids ID Plasmid Vector Type Promoter ...

...cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of...ARF GTPase family, information on each gene, and examples of plasmid types available for each gene. Click...gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids ...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375 181 Bacterial... and GEF family, information on each gene, and examples of plasmid types available for each gene. Click...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP) 55738 ...

...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight junctions VE-cadherin mApple* Michael Davidson 55165... that has a different emission wavelength. For example, if the marker protein is fused to EGFP, you may...with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria...Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection...Microtubules alpha-Tubulin mCherry Gia Voeltz 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru...

.... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found...visualize and track clones of cells over time. Examples of useful tools for studying cells that share ... Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish, ranging ...for studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...

...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...pEG545 - Worm Expression mMaple3 491 568 506 583 405 5.8 12 Monomer pmMaple3-CAM - Bacterial Expression...? Visit our Empty Backbones page or search our complete collection and filter by vector type, popularity...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...smURFP 642 670 32 3.2 39 min Prone to dimerization pLenti-smURFP - Mammalian Expression Return to top Near-Infrared...Monomer pmiRFP670-N1 - Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670...

...validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...targeting the PITCh donor plasmid. Note that the principle of the PITCh system is different from the conventional...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...nuclear and cytoplasmic proteins, which can then be depleted very rapidly after the addition of auxin to the...and can be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the...

...cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form . Please note that this ... (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and check the individual..., to order more than 2 µg of a single plasmid, multiple orders would need to be placed through our website...website. Every order will need to be completed independently including the check out and will require separate...I find cloning resources or scientific support? Please see our Addgene Protocols for resources you may...

...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...your plasmids! To get started with your deposit, please click here ....