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Showing: 41 - 57 of 57 results

  1. Antibody Plasmid Collection

    Type
    Collection
    ...Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin...Antibodies are large proteins made by the immune system that bind to an antigen, a particular part of a...bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies...-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic Human ...Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment...2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant... fragments in the form of scFvs A simple vector system to improve performance and utilisation of recombinant...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed for site-specific... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala...using CRISPR/Cas9 CRISPR...Orkin's and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional... functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based...repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ...

  5. Plasmid Collections

    Type
    Collection
    ...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology...pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ... Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human cells to...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski...four separate lentiviral plasmids A drug-inducible system for direct reprogramming of human somatic cells...Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4, Sox2, Klf4, and c-Myc...somatic cell. Sort the table by cell type, delivery system, species, or PI and then select the article link...

  7. Lentivirus Plasmids

    Type
    Collection
    ...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search...mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have been designed for increased...components 2nd versus 3rd generation lentiviral systems Viral production Frequently asked questions about...Envelope VSV-G recommended for the FELIX (FIV-based) system Nolan 22502 pCEP4-tat N/A Packaging Expression ...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...

  8. Distribution to Industry

    Type
    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium...

  9. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV ... has been circumvented with “helper-virus free systems,” which enable the production of infectious AAV... PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S...and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1... PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...been codon optimized for expression in mammalian systems. Other luciferases in our collection include Gaussia...optimized ( CBR2opt ) for mammalian expression systems. Each type of luciferase has advantages and disadvantages...half-life makes it too dim for many experimental systems. Firefly and Renilla luciferase are less-than-ideal...developed a wide range of luciferase-based plasmid systems for many different applications. Browse the Collection...proteostasis status in a wide range of experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet...plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV9-CRISPR knockout plasmid. Renilla luciferase is used to...

  11. Depositor Collections

    Type
    Collection
    ...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort...

  12. Synthetic Biology - Overview

    Type
    Collection
    ... Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo...describes the rational engineering of biological systems for novel functions. Addgene is working with leading...

  13. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink Lab Materials...Resources External Resources Teusink and Bruggeman Systems Biology Lab website (Link opens in a new window...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important ...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines. Features...transient double-selection of transfected cells, the system has successfully been employed for deletion mutagenesis...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope tag...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our...ultraID in mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of ... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...infection depends on two systems that work in concert: the innate and adaptive immune systems. Upon infection,... Lentiviral Expression (MuLE) System Kit : A modular and flexible system that can be used to construct... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene...infection, the immune system triggers several processes to activate cells to recognize and get rid of infectious...following exposure to a pathogen. The innate immune system is not specific to any particular pathogen. It ...Cytokines are molecules that enable cells of the immune system to communicate with each other across long distances...They trigger the defensive responses of the immune system upon infection with virus and, like other cytokines...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi Gan 186221 pBMN...Ward 178154 COQ2_Halo_N_allele COQ2 Halo Multiple system atrophy Michael Ward 178155 DAO_Halo_C_allele DAO...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 ... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy...
Showing: 41 - 57 of 57 results