...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh...

...plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors...

...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and...

... string of amino acids that preserves the sequence of your gene and of the fusion protein. Tag or... information about tags, including amino acid sequences, see our list of common tags . Back...

...work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum...

...dendrites under the control of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1...Cre-dependent expression; soma localization targeting sequence GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N...Cre-dependent expression; soma localization targeting sequence GENIE Project 174007-AAV9 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST...and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...

...recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then...

...domain, which targets the receptor to specific DNA sequences. Ligand binding triggers a conformational change...

...ability to optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...

...vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

...worm ( C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene...

...data and images from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell lines The...

...to amplification, enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins...

...and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. These ...

...The Rinehart lab synthesized 110,139 unique DNA sequences corresponding to every previously observed instance...

...expression vectors for insertion of custom gRNA target sequences....Cloning enzyme used for insertion of your gRNA sequence, such as BsbI or BsaI Cas9 (or other CRISPR) system...

...antibody’s identity to be easily confirmed via sequencing, and enables the variable region and constant...the DNA is released from the complex and can be sequenced and amplified for analysis. Overview of application...

...and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. In some...

...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence B PERB11.2...FAM3C family with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member...transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence b DKFZp686J10223...ERR3, FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76...Cas-Br-M (murine) ecotropic retroviral transforming sequence c CBL-3, CBL-SL, RNF57 CD247 CD247 molecule CD3... retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF from the labs...

...and (when possible) in vivo testing, and full sequencing of the final viral vector preparation. Systemic...