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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4 results for: Cas3

Showing: 1 - 4 of 4 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse CRISPR Plasmids By Function...Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates ...created using Cas3 have fewer off-target alterations and require only a single gRNA. Cas3 must be paired...using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using...combat this, some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single...directions from the target region, effectively turning Cas3 into a DNA “shredder” system that can cause large... targeted strand non-specifically along the way. Cas3 initiates a final double-strand break, either due...

  2. CRISPR Guide

    Type
    Collection
    ... Degrading DNA with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand...recruited to target DNA using a gRNA and recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading... strand is cleaved non-specifically by Cas3 along the way. Cas3 initiates a final DSB, either due to a...Figure 14: Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide...such as AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a ... enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems use a ...directions from the target region, effectively turning Cas3 into a DNA “shredder” system that can cause large...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR...
Showing: 1 - 4 of 4 results