... Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,... Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 ... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone..., N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (...

...of interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin... Plasmid Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible...Inducible Expression Additional Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi ...Curated Plasmid Collections Background Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea of ...

...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...